Researchers at the University of Minnesota have engineered a new synthetic biopathway that can more efficiently and cost-effectively turn agricultural waste, like corn stover and orange peels, into a variety of useful products ranging from spandex to chicken feed.
In the lab, researchers use engineered bacteria to brew green chemicals that can be used in a wide range of useful products. Photo Credit: University of Minnesota
The groundbreaking study was published today in the journal Nature Chemical Biology.
For years, researchers have been looking for more sustainable sources for the raw materials used to make the products we use every day. Recently, biomass made from corn or sugarcane is used in manufacturing of a wide range of non-food products from plastics to fuel. However, use of food to make inedible products is controversial because it affects the food supply and can elevate food prices.
In this study, researchers looked at turning inedible biological byproducts, that scientists call lignocelluloslic biomass, to produce useful products to avoid the “food versus chemical” purposes. They specifically looked at the process to use lignocellulosic biomass to produce butanediol (BDO) that is used to produce more than 1 billion pounds of spandex each year used in clothing and home furnishings. In 2010, it was estimated that spandex was used in 80 percent of all clothing.
To establish the platform pathway, researchers examined the gene sequences from bacteria and fungi that turn the biomass into tricarboxylic acid (TCA) intermediates. The researchers call this new metabolism “nonphosphorylative metabolism,” which enables the production of useful products from TCA cycle with less than five steps, compared to previous 10 steps. Less steps in the process resulted in a 70 percent higher yield in production and a process that is overall better for the environment.
What excited researchers the most was that this pathway could be used for more than just producing BDO for spandex. It can be used to produce a variety of useful products.
“We found that this new platform could be used to convert agricultural waste to chemicals that can be used for many other products ranging from chicken feed to flavor enhancers in food,” said the study’s lead researcher Kechun Zhang, a chemical engineering and materials science assistant professor in the University of Minnesota’s College of Science and Engineering.
“The pathway we developed was sustainable so it is better for the environment. This study is also one of the few examples of artificial metabolic pathways constructed so far,” Zhang added.
Zhang is a researcher in the National Science Foundation’s Center for Sustainable Polymers based at the University of Minnesota. His research has been funded primarily by the National Science Foundation and the University of Minnesota.
Other University of Minnesota students and researchers involved in this study include Yi-Shu Tai, Mingyong Xiong, Pooja Jambunathan, Jingyu Wang, Jilong Wang, and Cole Stapleton.
To read the full research paper, entitled “Engineering nonphosphorylative metabolism to generate lignocellulose-derived products,” visit the Nature Chemical Biology website.